Regulation of conjugal transfer by Lrp and Dam methylation in plasmid R100
- Eva Camacho 1
- Ana del Rosario Serna Gallego 1
- Josep Casadesús Pursals 1
- 1 Department of Genetics, Faculty of Biology, University of Seville, Seville, Spain
ISSN: 1618-1905
Año de publicación: 2005
Volumen: 8
Número: 4
Páginas: 279-285
Tipo: Artículo
Otras publicaciones en: International microbiology: official journal of the Spanish Society for Microbiology
Resumen
Conjugal transfer of the F-like plasmid R100 occurs at higher frequencies in the absence of DNA adenine methylation. Lower levels of R100-encoded FinP RNA were found in a Dam– host, suggesting that Dam methylation regulates FinP RNA synthesis. Lack of the leucine-responsive regulatory protein (Lrp) causes a decrease in R100 plasmid transfer, indicating that Lrp is an activator of R100- mediated conjugation. Hence, host-encoded regulators previously described for the Salmonella virulence plasmid (pSLT) seem to play analogous roles in R100. Repression of conjugal transfer in rich medium is an additional trait shared by R100 and pSLT. DNA sequence comparisons in regulatory loci support the view that R100 and pSLT are closely related. [Int Microbiol 2005; 8(4):279-285]