Registro electroencefalográfico de la actividad ictal en un modelo animal de ratones trangénicos app/ps1 y en pacientes con enfermedad de alzhemer

Resumen

Alzheimer’s disease (AD) is a common age-associated neurodegenerative disorder that is associated with an elevated risk for seizures that may be fundamentally connected to cognitive dysfunction. We used the APP/PS1 mice, model of AD, of 3-9 months of age to study the presence of seizure activity and to establish if the amyloid-β (Aβ) plaques may affect their generation. The spontaneous EEG of APP/PS1 transgenic mice were similar to wild-type mice. However, they revealed a major incidence of spontaneous seizures and lower threshold to evoke epileptic discharges than control animals when pentylenetetrazole (0.5%) was injected. We also found a correlation between the frequency of spontaneous seizures and the number of Aβ plaques. Application of N-(2-chloroethyl)-N-ethyl-bromobenzylamine (50 mg/kg) generated Aβ plaques in the cortex and seizure activity appeared. Taken together these data indicate that deposit of Aβ plaques may be responsible for the epileptic seizures recorded in the APP/PS1 mice. Our findings suggest that a careful EEG study in AD patients could help to diagnosis this pathology Keywords: interictal spikes, Aβ plaques, EEG, pentylenetetrazole, DSP4, APP/PS1 Mice