Intervención con ejercicio físico en un modelo murino de neuroblastoma de alto riesgo

Abstract

Our purpose was to evaluate the effects of a physical exercise program in a murine model of high-risk neuroblastoma. Thirty-five wild-type TH-MYCN male mice were allocated in one of two groups (exercise, n=17; sedentary, n=16) after orthotopic inoculation of high-risk neurobiastoma tumor cells. The exercise intervention lasted five weeks and included aerobic and strength exercises. Clinical evolution and survival were assessed daily in all the mice, and aerobic capacity and muscle strength were analyzed before and after the exercise intervention. In turn, tumor volume and weight, metastasis and different measures related to the tumoral microenvironment were assessed at the end of the intervention period. The maximal aerobic capacity in the sedentary group decreased significantly (pSO.05) during the study period, whereas it remained unchanged in the exercise group. As for intratumoral variables, the exercise group showed greater levels of caspase 3 and vascular endothelial growth factor receptor 2 expression (psO.05). There were also significant differences in peripheral blood 4-1BB CD8+ receptors (pS0.05), with the sedentary group showing a more marked decrease of these receptors throughout the study compared with the exercise group. In conclusion, a supervised exercise program intervention in a high-risk neuroblastoma murine model is safe and can induce some significant benefits, providing new scientific evidence that validates the future use of intrahospital physical exercise programs.